Abstracts - Alana Kelyene Pereira


Alana Kelyene Pereira1, Doraí Periotto Zandonai1, Eli Fernando Pimenta, Maria de Fátima das Graças Fernandes1, Paulo Cesar Vieira1, Moacir Forim1, Carlos Pagnocca2, João Batista Fernandes1

1 Universidade Federal de São Carlos; alanakelyene@gmail.com

2 UNESP – Rio Claro

* Correspondence: alanakelyene@gmail.com

The huge Brazilian economy related to the development of agriculture is very important for its world population. Taking in account the climatic, natural, and geographical diversity, this country has 388 million fertile arable and high productivity lands. Leaf- cutter ant (Attini: Formicidae) are important pests in plantation of Pinus and Eucaliptus, and they live in an obligate mutualism with fungi Leucoagaricus gongylophorus that is cultivated as food. The complex ecology, evolution and chemistry of symbiotic interactions present in ants gardens opens the creativity to study. The symbiosis currently contains five identified and characterized symbiots: Attine ants, the fungi, cultivar- attacking microfungi of the genus Escovopsis, antibiotic-producing bacteria by the genus Pseudonocardia, and a black yeast parasitizing the ant-Pseudonocardia mutualism. All these interactions need more knowledge of different study areas. This work aims to evaluate the interactions of the symbiote fungi, Leocoagaricus gongylophorus, with black yeast, isolated recently from the garden, in co-culture, analyzing the differences in chromatograms, MS and NMR spectra. Fungi and yeast of genus Aureobasidium, Exophiala, Phialophora e Pyrenochaeta were isolated from the tegument of leaf-cutter ant and identified by CEIS – UNESP Rio Claro. The process of extraction of compounds was made with the entire Petri plate, using methanol/ dichloromethane/ethyl acetate (1:2:3). Theses samples were submitted to analysis of HPLC-DAD with a C18 column (Macherey-Nagel® 150 x 4.60 mm, particles de 5 μm). The mono-cultures of the fungi were made in liquid medium, and the mycelium was extracted with methanol/ethanol (1:1) during 5 days. Through the analysis of chromatograms, it was verified the decreasing of the number of chromatografics peaks when compared with the mono co- culture, suggesting the inhibition of production of some metabolites. In the Petri plates, it is possible to see the zone of inhibition, due to the alteration of colors or morphology of the culture. The 1H NMR spectra of the extracts of mycelium of fungi in liquid medium presented high complexity, suggesting the presence of polysaccharides (signals of high intensity in the region of anomeric hydrogens and bounded to hydroxyls of polysaccharides). The samples are been analyzed by UPLC-MS. Studies related to co- culture systems are very important to understand the biological progress in the nature. Initial analysis show that this promising approach constitutes a strategic method to highlight new structures and, possibly, new skeletons with relevant biological activity and potentially new modes of action in the field of drug discovery and ants control.

FAPESP proc. 2012/25299-6 e 2016/12304-2.